Work zone crashes have always drawn public attention. A number of fatalities are recorded every year nationwide within work zone areas. Most existing countermeasures have been dedicated more to the advance warning areas, transition areas, and activity areas of work zone, than the termination areas, where drivers might play less attention to safety threats. In this study, the vehicle-to-vehicle communication based left turn warning system was applied at a work zone termination area, which is immediately followed by a T-intersection. The work zone is located on the minor road side, while left turn vehicles will be appearing from the major street through the said T-intersection. A smart phone application was designed using Android coding system to provide several types of warning messages to drivers. Corresponding scenarios were designed in a driving simulator, and 20 subjects were recruited to participate in the simulation test followed by a questionnaire survey. The subjects received a warning message when driving to the termination area of a work zone on the coming left turn vehicles. Twenty test drivers’ driving speed, acceleration rates, and break reaction distance to the warning messages were studied in four different scenarios. Results show that the smartphone application has a great impact on driving behaviors, especially the female voice and the beep tone warning, which are recommended for possible field tests. Besides, the developed smartphone applications can be further updated for practical applications of similar needs.