The objective of this project is to evaluate MoDOT’s alternate lane shift sign configuration for work zones. The single sign proposed by MoDOT provides the traveler with enough information to let them know that all lanes are available to shift around the work zone, whereas the MUTCD signs require drivers to see two signs. This research simulation project evaluates the drivers’ lane shifting performance and acceptance of the alternate lane shift sign proposed by MoDOT to be used on work zones as compared to the MUTCD lane shift signs. Based on the study results, no difference was observed between MUTCD lane shift sign and MoDOT lane shift sign lane shift patterns with respect to driving patterns. In summary, statistical data analysis clearly demonstrated that there was not a noticeable, statistical difference between lane change patterns of drivers in the MoDOT alternate signs with MUTCD signs in the work zone.